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Image Search Results
Journal: Frontiers in Immunology
Article Title: Interleukin-4 Inhibits Regulatory T Cell Differentiation through Regulating CD103+ Dendritic Cells
doi: 10.3389/fimmu.2017.00214
Figure Lengend Snippet: Interleukin (IL)-4 inhibits indoleamine 2,3-dioxygenase (IDO) expression during dendritic cells differentiation . Bone marrow-derived DCs (BMDCs) were propagated from bone marrow cells in GM-CSF (10 ng/ml) and different dose of IL-4 (2, 5, and 10 ng/ml) until day 7. Monocyte-derived DCs (MoDCs) were generated from CD14+ monocytes in GM-CSF (50 ng/ml) and various dose of IL-4 (5, 10, and 20 ng/ml) until day 6. After differentiation, total protein from BMDCs and MoDCs were extracted and subjected to assess IDO expression by immunoblot. All data are representative of one of the three independent experiments.
Article Snippet: CD14+ monocytes were purified from PBMCs using positive selection with
Techniques: Expressing, Derivative Assay, Generated, Western Blot